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Review PCR instrument: LightCycler ® 96

Roche LightCycler® 96 System was relased  in november 2012 at American Society for Human Genetics annual meeting in San Fransisco. This is a mid-price segment of the market qPCR Machine dedicated for life science research only.

LightCycler 96 Roche PCR machine reviewRoche instruments are well known for an user-friendly interface.This platform comes with a redesign software that is designed to provide guided navigation. Sofware comes free of charge and implements the real time PCR data markup language, or RDML file format witch become very popular on global level as a universal file format for real time pcr data. A fact proved by a long list of supporters of this standardization.

The new platform is dedicated to both high-end research customers and more casual users (instructors in teaching laboratories for students) in the mid-price segment of the market (around 30,000 US $).  The 96 well block format represents more than 50% of the market, and is dedicated for users that demand more automation.

Key features

Silver Block
The silver block designed to ensure a high degree of temperature homogeneity and uniformity thus improving data consistency and accuracy ( +/- 0.2° Celsius from the target temperature).

Optical fibers
The 96 individual glass optical fibres ensuring equal and simultaneous data capture from all 96 wells. The system is also calibration-free, as it does not require a passive reference dye.

With the new LightCycler 96, Roche complete the portfolio which also includes the following instruments:

  • LightCycler 480 (switch between 96 and 384 well plate)
  • LightCycler 1536 (the most expensive,pharmaceutical research, discovery, and quality control)
  • LightCycler Nano (32 well plates introduced in 2011, sold to academic users )

PCR Thermal Cycler Open Source Solution

How about OpenPCR ?

An open source solution for development of PCR thermal cyclers
Open source movement is an old hat with respect to computer software but since the last two years there is a similar trend among molecular biologists interested in DNA testing. In fact, there is growing support for a project that provides information about the elaborate technology behind PCR thermal cyclers. There are encouraging signs that open source constructed PCR thermal cyclers could become a reality soon.

How much would OpenPCR cost ?

The Californian biologists Tito Jankowski and Josh Perfetto took matters into their own hands and collected more than $10,500 for their project OpenPCR. Here they launch an open source PCR machine that is financially attractive enough to inspire scientists to develop their own thermal cycler. Open PCR aims to provide this open source PCR thermal cycler for less than $400 – an astonishing price for an instrument that usually costs more than $4,000!

Jankowski and Perfetto state that their project OpenPCR will give interested users the possibility to undertake various experiments involving DNA. For example, food and plants could be tested for the presence of genetically modified organisms and even human genomes can be investigated.

How to become a part of OpenPCR ?

Interested in becoming part of the project or in the PCR thermal cycler itself? Just visit the OpenPCR kickstarter page to learn more.

What is a Thermal Cycler?

A PCR thermal cycler is a high tech laboratory instrument that is used for DNA amplification via Polymerase Chain Reaction (PCR). This makes it to one of the centerpieces of a molecular biology lab. If you look at the thermal cycler (also known as PCR machine, thermocycler or DNA amplifier) objectively, it mainly consists of a thermal block with a grid of holes, which holds the reaction tubes with PCR probes. The thermal block can increase or decrease temperature very rapidly, temperature steps and their duration can be programmed.

Why and how was the Thermal Cycler created?

In the beginning of PCR research a modified DNA polymerase from E. coli – the Klenow fragment – was used. As this enzyme was inactivated by high temperatures in the amplification process and hence became useless for further amplification. This made the process of PCR very cumbersome and time consuming because the enzyme had to be replaced after every single step of the process. Even the invention of automated pipettors was only a minor improvement because in this version open tubes had to be used, which increased the risk of evaporation of the small PCR samples.

The utilization of heat-resistant DNA polymerase from Thermus aquaticus made the final breakthrough possible. Instrumental design of PCR thermal cyclers could be simplified. Temperature control was performed by the use of oil baths with constant temperature, more elaborate models were made from silver in order to ensure rapid temperature changes and uniform temperature distribution throughout the complete thermal block.

How does a Thermal Cycler work today?

Modern PCR thermal cyclers feature a thermoelectric heat pump that provides the possibility for reliable temperature control with the advantage that no constant temperature bath is required anymore. Another improvement are heated lids, which fit tightly against the top of the PCR reaction tubes in order to prevent evaporation of water from the PCR samples that would otherwise condensate on the tube caps. This process would increase the salt concentration of the PCR sample dramatically and to avoid this, PCR tubes had to be sealed with mineral oil in the past. Today PCR thermal cyclers with multiple thermal blocks are available, which enable the user to perform several PCR reactions according to different protocols at the same time. High-end thermal cyclers come with a temperature gradient function that can be very useful when the suitability of annealing temperatures for primers has to be tested.

Current PCR thermal cycler are technical masterpieces but we may essentially assume that new improvements will be launched during the next years.